Whole - genome restriction mapping by “ subhaploid ” - based RAD 1 sequencing : an efficient and flexible approach for physical mapping 2 and genome scaffolding 3
نویسندگان
چکیده
Whole-genome restriction mapping by “subhaploid”-based RAD 1 sequencing: an efficient and flexible approach for physical mapping 2 and genome scaffolding 3 Jinzhuang Dou, Huaiqian Dou, Chuang Mu, Lingling Zhang, Yangping Li, 4 Jia Wang, Tianqi Li, Yuli Li, Xiaoli Hu, Shi Wang, and Zhenmin Bao 5 6 * Key Laboratory of Marine Genetics and Breeding (Ocean University of China), 7 Ministry of Education, Qingdao 266003, China 8 † Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 9 National Laboratory for Marine Science and Technology, Qingdao 266237, China 10 ‡ Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for 11 Marine Science and Technology, Qingdao 266237, China 12 13 1 Present address: Department of Computational and Systems Biology, Genome 14 Institute of Singapore, Biopolis 138672, Singapore 15 2 These authors contributed equally to this work. 16 3 Corresponding authors. 17 18 Reference numbers for data generated by this study and deposited in the SRA 19 database: 2b-RAD data, SRP068747; Illumina MiSeq and PacBio WGS data, 20 SRP068748 & SRP068751. 21 22
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Whole-Genome Restriction Mapping by “Subhaploid”-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding
Assembly of complex genomes using short reads remains a major challenge, which usually yields highly fragmented assemblies. Generation of ultradense linkage maps is promising for anchoring such assemblies, but traditional linkage mapping methods are hindered by the infrequency and unevenness of meiotic recombination that limit attainable map resolution. Here we develop a sequencing-based "in vi...
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